Event

In person & remote options available:

https://upenn.zoom.us/j/97682459971

Meeting ID: 976 8245 9971

 

Dr. Martin Wuehr

Princeton University

Proteomic Clues to Cell Organization

[Dr. Wuehr] will talk about recent advances in quantitative multiplexed proteomics that improve data quality while being compatible with simpler and easier-to-access instrumentation. [Dr. Wuehr] will also discuss two case studies where we have successfully applied the developed technologies to obtain a systems-level understanding of cellular organization. First, we quantified the partitioning of proteins between the nucleus and cytoplasm in the developing embryo. We find that the access of maternally deposited nuclear proteins to the genome is temporally ordered via importin affinities, thereby timing the expression of downstream targets. Second, we studied the organization of native cytoplasm in biomolecular condensates (BMCs) on a global scale. We find that at least 15% of the proteome organizes itself via BMCs and that this organization predominantly occurs at the mesoscale of ~ 100nm.